Barzda, Virginijus

Ph.D. | Professor | Physics | Biophysics and Physical Chemistry

Contact Information

905 828 3821
905 828 5425
Mailing Address: 
3359 Mississauga Road
Postal Code: 
L5L 1C6
Picture of Virginijus Barzda


A picture of Barzda's research

Our research focuses on the development of new imaging modalities for multimodal non linear microscopy. Currently we employ second harmonic generation, third harmonic generation and multiphoton excitation fluorescence contrast mechanisms, which are generated simultaneously after femtosecond pulse excitation. Our microscopes are tailored to image non-invasively live cells and subcellular organelles. We investigate intracellular dynamics in photosynthetic systems, research contractility mechanisms in cardiac and skeletal muscle cells, and study structural organization of cancerous tissue.



Golaraei, A., Kontenis, L., Karunendiran, A., Stewart, B.A., and Barzda, V. (2020) Dual- and single-shot susceptibility ratio measurements with circular polarizations in second-harmonic generation microscopy, J. Biophotonics, e201960167, 1-9.

Zhao, H., Cisek, R., Karunendiran, A., Tokarz, D., Stewart, B.A. and Barzda, V. (2019) Live imaging of contracting muscles with wide-field second harmonic generation microscopy using a high power laser, Biomed. Opt. Express, 10(10):5130-5135.

Mirsanaye, K., Golaraei, A., Habach, F., Zurauskas, E., Venius, J., Rotomskis, R. and Barzda, V. (2019) Polar organization of collagen in human cardiac tissue revealed with polarimetric second-harmonic generation microscopy, Biomed. Opt. Express, 10(10):5025-5030.

Golaraei, A., Kontenis L., Mirsanaye, K., Krouglov, S., Akens, M.K., Wilson, B.C. and Barzda, V. (2019) Complex susceptibilities and chiroptical effects of collagen measured with polarimetric second-harmonic generation microscopy, Sci. Reports, 9:12488.

Tokarz, D., Cisek, R., Joseph, A., Golaraei, A., Mirsanaye, K., Krouglov, S., Asa, S.L., Wilson, B. and Barzda, V. (2019) Characterization of pancreatic cancer tissue using multiphoton excitation fluorescence and polarization-sensitive harmonic generation microscopy, Frontiers in Oncology, 9: Article 272.

Krouglov, S. and Barzda, V. (2019) Three-dimensional nonlinear Stokes–Mueller polarimetry, J. Opt. Soc. Am. B, 36(2):541-550.

Golaraei, A., Mirsanaye, K., Ro, Y., Krouglov, S., Akens, M.K., Wilson, B.C. and Barzda, V. (2018) Collagen chirality and three-dimensional orientation studied with polarimetric second-harmonic generation microscopy, J. Biophotonics, e201800241, 1-9.

Fung, K.L.B., Samim, M., Gribble, A., Barzda, V. and Vitkin, I.A. (2018) Monte Carlo simulation of polarization-sensitive second-harmonic generation and propagation in biological tissue, J. Biophotonics, e201800036, 1-11.

Cisek, R., Tokarz, D., Kontenis, L., Barzda, V. and Steup, M. (2018) Polarimetric second harmonic generation microscopy: An analytical tool for starch bioengineering, Starch/Stärke, 70: 1700031 1-15 (review).

Karunendiran, A., Cisek, R., Tokarz, D., Barzda, V. and Stewart, B.A. (2017) Examination of Drosophila eye development with third harmonic generation microscopy, Biomed Opt. Express, 8(10):4505-4513.

Burke, M., Golaraei, A., Atkins, A., Akens, M., Barzda, V. and Whyne, C. (2017) Collagen fibril organization within rat vertebral bone modified with metastatic involvement, J. Struct. Biol. 199:153-164.

Krouglov, S. and Barzda, V. (2017) Modified Gibbs’s representation of rotation matrix, Ann. Fond. Louis de Broglie, 42(2):1-15.

Kontenis L., Samim, M., Krouglov, S. and Barzda, V. (2017) Third-harmonic generation Stokes-Mueller polarimetric microscopy, Opt. Express, 25(12):13174-13189.

Samim, M., Krouglov, S., James, D. F. and Barzda, V. (2016) Characterization of heterogeneous media using nonlinear Stokes–Mueller polarimetry, J. Opt. Soc. Am. B, 33: 2617-2625.

Golaraei, A., Kontenis, L., Cisek, R., Tokarz, D., Done, S. J., Wilson, B. C., and Barzda, V. (2016) Changes of collagen ultrastructure in breast cancer tissue determined by second-harmonic generation double Stokes-Mueller polarimetric microscopy, Biomed Opt. Express, 7: 4054-4068.

Samim, M., Krouglov, S. and Barzda, V. (2016) Three-photon Stokes-Mueller polarimetry, Phys. Rev. A., 93: 033839-1-13.

Samim, M., Krouglov, S. and Barzda, V. (2016) Nonlinear Stokes-Mueller polarimetry, Phys. Rev. A., 93:013847-1-6.

Kontenis, L., Samim, M., Karunendiran, A., Krouglov, S., Stewart, B. and Barzda, V. (2016) Second harmonic generation double Stokes Mueller polarimetric microscopy of myofilaments, Biomed. Opt. Express, 7:559-569.

Cui, L., Tokarz, D., Cisek, R., Ng, K.K., Wang, F., Chen, J., Barzda, V. and Zheng, G. (2015) Organized aggregation of porphyrins in lipid bilayer for third harmonic generation microscopy, Angew. Chem., 127:14134-14138.

Cisek, R., Tokarz, D., Steup, M., Tetlow, I.J., Emes, M.J., Hebelstrup, K.H., Blennow, A. and Barzda, V. (2015) Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration, Biomed. Opt. Express, 6: 3694-3700.

Tokarz, D., Cisek, R., Golaraei, A., Asa, S.L., Barzda, V. and Wilson B.C. (2015) Ultrastructural features of collagen in thyroid carcinoma tissue observed by polarization second harmonic generation microscopy, Biomed. Opt. Express, 6: 3475-3480.

Samim, M., Krouglov, S. and Barzda, V. (2015) Double Stokes Mueller polarimetry of second harmonic generation in ordered molecular structures, J. Opt. Soc. Am. B, 32: 451-461.

Cisek, R., Tokarz, D., Krouglov, S., Steup, M., Emes, M.J., Tetlow, I.J. and Barzda, V. (2014) Second harmonic generation mediated by aligned water in starch granules, J. Phys. Chem. B, 118: 14785-14794.

Cisek, R., Tokarz, D., Hirmiz, N., Saxena, A., Shik, A. Ruda, H. and Barzda, V. (2014) Crystal lattice determination of ZnSe nanowires with polarization-dependent second harmonic generation microscopy, Nanotechnology, 25: 505703+01-09.

Golaraei, A., Cisek, R., Krouglov, S., Navab, R., Niu, C., Sakashita, S., Yasufuku, K., Tsao, M.S., Wilson, B. C., and Barzda, V. (2014) Characterization of collagen in non-small cell lung carcinoma with second harmonic polarization microscopy, Biomed. Opt. Express, 5: 3562- 3567.

Tokarz, D., Cisek, R., El-Ansari, O., Espie, G. S., Fekl, U., and Barzda, V. (2014) Organization of astaxanthin within oil bodies of Haematococcus pluvialis studied with polarization-dependent harmonic generation microscopy, PLOS ONE, 9: e107804-1-8

Samim, M., Prent, N., Dicenzo D., Stewart, B. and Barzda, V. (2014) Second harmonic generation polarization properties of myofilaments, J. Biomed. Opt. 19: 056005-1-056005-6.

Piunno, P. A. E., Boyd, C., Barzda, V., Gradinaru, G. C., Krull, U. J., Stefanovic, S. and Stewart, B. (2014) The advanced interdisciplinary research laboratory: A student team approach to the fourth-year research thesis project experience, J. Chem. Educ., 91:655-661.

Sandkuijl, D., Kontenis, L., Coelho, N. M., McCulloch, C. and Barzda, V. (2014) Interferometric backward third harmonic generation microscopy for axial imaging with accuracy beyond the diffraction limit, PLOS ONE, 9:e94458 1-6.

Tokarz, D., Cisek, R., Krouglov, S., Kontenis, L., Fekl, U., and Barzda, V. (2014) Molecular organization of crystalline β‑carotene in carrots determined with polarization-dependent second and third harmonic generation microscopy, J. Phys. Chem, B., 118:3814−3822.

Samim, M., Sandkuijl, D., Tretyakov, I., Cisek, R. and Barzda, V. (2013) Differential polarization nonlinear optical microscopy with adaptive optics controlled multiplexed beams, Int. J. Mol. Sci., 14:18520-18534.

Tokarz, D., Cisek, R., Fekl, U. and Barzda, V. (2013) The molecular second hyperpolarizability of the light-harvesting chlorophyll a/b pigment−protein complex of photosystem II, J. Phys. Chem, B., 117:11069−11075.

Sandkuijl, D, Tuer, A. E., Tokarz, D., Sipe, J. E. and Barzda, V. (2013) Numerical second- and third-harmonic generation microscopy, J. Opt. Soc. Am. B, 30:382-395.

Tokarz, D., Cisek, R., Prent, N., Fekl, U. and Barzda, V. (2012) Measuring the molecular second hyperpolarizability in absorptive solutions by the third harmonic generation ratio technique, Anal. Chim. Acta, 755:86-92.

Tuer, A. E., Akens, M. K., Krouglov, S. Sandkuijl, D., Wilson, B. C., Whyne, C. M. and Barzda, V. (2012) Hierarchical Model of Fibrillar Collagen Organization for Interpreting the Second-Order Susceptibility Tensors in Biological Tissue, Biophys J. 103:2093-2105.

Tokarz, D., Cisek, R., Garbaczewska, M., Sandkuijl, D., Qiu, X. P., Stewart, B., Levine, J. D., Fekl, U. and Barzda, V. (2012) Carotenoid Based Bio-Compatible Labels for Third Harmonic Generation Microscopy, Phys. Chem. Chem. Phys., 14: 10653-10661.

Prent, N., Tuer, A., Cisek, R., Samim, M., Krouglov, S. and Barzda, V. (2012) Polarization-in Polarization-out second harmonic generation microscopy in striated muscle, Proc. Int. School Phys. "Enrico Fermi", SIF, Bologna and IOS Press, Amsterdam.

Tuer, A., Krouglov, S., Prent, N., Cisek, R., Sandkuijl, D., Yasufuku, K., Wilson, B.C. and Barzda, V. (2011) Nonlinear Optical Properties of Type I Collagen Fibers Studied by Polarization Dependent Second Harmonic Generation Microscopy, J. Phys. Chem. B, 115:12759-12769.

Cisek, R., Barzda, V., Ruda, H. E. and Shik, A. (2011) Nonlinear Optical Properties of Semiconductor Nanowires, Ieee J. Select. Topic. Quant. Electron., 17:915-921 (invited paper).

Tuer, A., Krouglov, S., Cisek, R., Tokarz, D. and Barzda, V. (2011) Three-dimensional visualization of the first hyperpolarizability tensor, J. Comp. Chem. 32:1128-1134.

Samim, M, Cisek, R., Sandkuijl, D., Musikhin, S., Prent, N. Stewart, B. and Barzda, V. (2010) Towards fast 3D microscopy: multiple depth focusing for video-rate imaging, Phys Canada, 66:183-185.

Tokarz, D, Tuer, A., Cisek, R., Krouglov, S. and Barzda, V. (2010) Ab initio calculations of the linear and nonlinear optical properties of amino acids, J. Phys, 256:012015-1-5.

Sandkuijl, D., Cisek, R., Major, A., and Barzda, V. (2010) Differential microscopy for fluorescence-detected nonlinear absorption linear anisotropy based on a staggered two-beam femtosecond Yb:KGW oscillator, Biomed. Opt. Express, 1:895-901.

Tuer, A., Tokarz, D., Prent, N., Cisek, R., Alami, J., Dumont, D., Bakueva, L., Rowlands, J. and Barzda, V. (2010) Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections, J. Biomed. Opt. 15:0260181-02601819.