Barzda, Virginijus

Ph.D. | Professor | Physics | Biophysics and Physical Chemistry

Contact Information

905 828 3821
905 828 5425
Mailing Address: 
3359 Mississauga Road
Postal Code: 
L5L 1C6
Picture of Virginijus Barzda


A picture of Barzda's research

Our research focuses on the development of new imaging modalities for multimodal non linear microscopy. Currently we employ second harmonic generation, third harmonic generation and multiphoton excitation fluorescence contrast mechanisms, which are generated simultaneously after femtosecond pulse excitation. Our microscopes are tailored to image non-invasively live cells and subcellular organelles. We investigate intracellular dynamics in photosynthetic systems, research contractility mechanisms in cardiac and skeletal muscle cells, and study structural organization of cancerous tissue.



Barzda V. (2008) Non-linear Contrast Mechanisms for Optical Microscopy, In: Biophysical Techniques in Photosynthesis II, Eds: Aartsma T. and Matysik J., pp 35-54.

Greenhalgh, C., Prent, N., Green, C., Cisek R., Major, A., Stewart, B. and Barzda, V., (2007) The influence of semicrystalline order in anisotropic bands on the second harmonic generation efficiency of myocytes, Applied Optics, 46:1852-1859.

Major A., Cisek R., and Barzda V. (2006) Femtosecond Yb:KGd(WO4)2 laser oscillator pumped by a high power fiber-coupled diode laser module, Optics Express, 14:12163-12168.

Major, A., Barzda, V., Piunno, P. A. E., Musikhin, S., Krull, U. J. (2006) An extended cavity diode-pumped femtosecond Yb: KGW laser for applications in optical DNA sensor technology based on fluorescence lifetime measurements, Optics Express, 14: 5285-5294.

Barzda, V., Greenhalgh, C., Aus der Au, J., Elmore, S., Van Beek, J.H.G.M., and Squier, J. (2005) Visualization of mitochondria in cardiomyocytes by simultaneous harmonic generation and fluorescence microscopy, Optics Express, 13: 8263-8276.

Cseh, Z. Vianelli, A. Rajagopal, S. Krumova, S. Kovacs, L. Papp, E. Barzda, V. Jennings, R. Garab, G. (2005) Thermo-optically induced reorganizations in the main light harvesting antenna of plants. I. Non-arrhenius type of temperature dependence and linear light-intensity dependencies, Photosynth. Res. 86: 263-273.

Barzda, V., Ionov, M., Van Amerongen, H., Gussakovsky, E. E. and Shahak, Y, (2004) The effect of pea chloroplast alignment and variation of excitation wavelength on the circularly polarized chlorophyll luminescence, J. Fluorescence, 14: 207-216.