Confocal Microscope, Zeiss LSM 800
Confocal Microscope, Zeiss LSM 880
Confocal Spinning Disc, Quorum Technologies X-Light V2
Epifluorescence microscope, Nikon Eclipse 50i
JEOL NeoScope JCM-5000
Confocal Microscope, Zeiss LSM 800

The Zeiss LSM 800 is a laser scanning microscope system equipped with an inverted fluorescence microscope, four laser lines, highly sensitive GaAsP detectors, an Airyscan detector, and a transmitted light detector. It is capable of simultaneously acquiring 4 fluorescent images and 1 DIC image in a single sample scan, and is able to do three dimensional fluorescence imaging. Technical specifications are as follows.
Microscope
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Observer Z1
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High-end fully motorized inverted microscope
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Remote LCD touch screen allowing for remote control of all motorized components
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Motorized Z motor with 10nm reproducibility and customizable variable speed focus control
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Motorized XY scanning stage, minimum travel range of 130x85mm, with <1µm reproducibility. Capable of holding slides, labtek chambers, 35mm dishes.
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Motorized light path control, condensor, illumination shutters, reflector turret, filter cubes
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Hardware-based focus stabilization, capable of being used for multiple xy locations in combination with z-stack, time course, and spectral imaging, in order to optimize acquisition from live cell experiments
Confocal
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XY image resolution of 6000x6000 pixels
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Spectral detection with a spectral resolution as low as 1nm, with a range of 400-720nm (detection available >720)
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Spectral un-mixing of up to 10 fluorophores, with 20 spectral channels
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360 degree rotation of scan field
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Multi-Region of Interest FRAP capability, with ability to vary laser power between FRAP locations
Objectives
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EC Plan-Neofluar 10x/0.5
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Plan Apochromat 20x/0.8 NA
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Plan Apochromat 40x/1.4 NA oil immersion
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Plan Apochromat 63x/1.4NA oil immersion
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Differential Interference Contrast for all objectives
Lasers
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Full complement of solid state lasers, with the following wavelengths and minimum powers, or equivalent. Laser line intensity variable from 1-100% in 0.1% increments, with full linear response.
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405nm (5mw)
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488nm (10mw)
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555nm (10mw)
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639mw (5mw)
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Detectors
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Capable of simultaneously acquiring 4 fluorescent images and 1 DIC image in a single sample scan
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Two internal high-sensitivity low noise Gallium Arsenide Phosphide detectors (GaAsP)
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One high-sensitivity low noise super resolution detector (Airyscan)
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Single transmitted light detector for DIC imaging in parallel with confocal
Widefield Fluorescence
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Filters for DAPI, GFP, and Cy3, or equivalent dyes
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Alignment-free extended long life (20000hr) LED fluorescence light source
Table
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Actively dampened isolation table
Computer
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Windows 7 professional
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Confocal Operating Software Zen 2.3
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Confocal Microscope, Zeiss LSM880

The Zeiss LSM 880 is a laser scanning microscope system equipped with an upright fluorescence microscope, four conventional laser lines, and a tunable insight X3 laser for 2-photon imaging. It has GaAsP detectors, an Airyscan detector, and Big-2 detector for 2-photon imaging. The system delivers super-resolution imaging and fast scanning speed, and is able to do three dimensional imaging. The 2-photo imaging mode, with a long working distance objective, is capable of acquiring optical sections of deep tissue layers. Technical specifications are as follows:
Microscope
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Examiner Z1
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Upright
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Objectives are changed manually
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Z-Piezo stage
Objectives
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EC PlnN 10X /0.3 DICI, dry
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W Pln Apo 20x/ 1 w DICIII, water
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LD LCI plnApo 25x/0.8 W DICII, water /Glycerol with or without cover slip/ oil with cover slip
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Pln Apo 63x/1.4 Oil DIC II, with cover slip
Lasers
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Diode 405nm
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Argon 458nm, 488nm, 514nm
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DPSS 561nm
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HeNe 633
Detectors
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Gallennium Arsenide Phosphide detectors (GaAsP)
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One high-sensitivity low noise super-resolution detector (Airyscan)
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BiG-2 GaAsP detector for 2-photon imaging
2-Photon
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InsightX3 Tunable laser; 690-1080 nm
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Emission filters for green (500-550 nm) and red (575-610 nm)
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BIG-2 GaAsP detector
Wild field Fluorescence
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Filter sets available:
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DAPI, Zeiss filter set 49
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Alexa 488, Zeiss filter set 38
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Alexa 555, Zeiss Filter set 43
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Light source
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X-cite 110 LED
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Table
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Newport ST Series
Computer
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Windows 7
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Operating Software Zen 2.3 SP1
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Confocal Spinning Disc, Quorum Technologies X-Light V2

The acquisition system is an X-Light V2 spinning disk confocal with an LDI-7 laser engine, supplied and integrated by Quorum Technologies. The system is equipped with a stage-top live cell incubator for live cell imaging in 35 mm dishes, or 12-, 24-, 48-, or 96-well plates. Imaging with a confocal spinning disk is useful for high-speed imaging of living cells expressing fluorescent proteins or stained with membrane-permeable synthetic dyes. Photo-bleaching and photo-toxicity are reduced with spinning disk microscopy. Technical specifications are as follows:
Microscope
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Leica DMi8 inverted microscope with an ASI motorized stage
Objectives
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10x, dry
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20x, dry, long working distance
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40x, dry
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63x, water
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63x, oil
Lasers
Excitation wavelengths:
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405 nm
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445 nm
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470 nm
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520 nm
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528 nm
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555 nm
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640 nm
Emission filters:
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460/50m
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525/50m
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610/75m
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700/75m
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528/640m (dual Green Red)
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445/520m (dual CFP YFP)
Camera
The system is installed with two cameras, mounted via a twin-cam system.
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Prime HS: sCMOS camera, for high resolution.
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QuantEM: EM-CCD, for high sensitivity applications.
Widefield Fluorescence
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Filter sets available:
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DAPI; material number 11525304; Excitation: 350/50; Emission 460/50; Dichroic: 400
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Y5, size P, material number 11525312; Excitation: 620/60; Dichroic: 660; Emission: 700/75
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FITC, material number 11525307; Excitation: 480/40; Dichroic: 505; Emission: 527/30
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RHOD, material number 11525308; Excitation: 546/10; Dichroic: 560; Emission: 585/40
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Lamp:
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X-Cite 110 LED, Lumen Dynamics
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Live Cell incubation
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Chamlide TC-W stage-top incubator
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FC-5N CO2/Air Mixer
Computer
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Dell 7820 running Windows 10
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Acquisition software Metamorph v7.10
Data Analysis Station
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Running imaging analysis software Volocity, managed by network license server
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Epifluorescence Microscope Nikon Eclipse 50i

A conventional upright fluorescence microscope, for samples on slide with cover slip.
Condenser
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Phase contrast, NA 0.9, dry
Objectives
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Nikon Plan uw 1x/0.04
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Nikon Plan Fluo 4x /0.13
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Nikon Plan Fluo 10x/0.25
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Nikon Plan 20x/0.04
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Nikon Plan Fluo 100x1.30 Oil
Fluorescence Filters
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ET DAPI
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ET GFP
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ET RsRed
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UV-2A
Lamp
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X-Cite 120
Imaging Software
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NIS-Elements BR3.0
Camera
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Nikon DS-Ri1
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JEOL NeoScope JCM-5000

The NeoScope is a benchtop scanning electron microscope (SEM) that can rapidly acquire images with high magnification, high resolution, and a large depth of field. No special sample preparation (eg. coating) is required.
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X10 -- 20,000 magnification
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Easy automatic or manual control of focus, contrast, and brightness
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High and low vacuum modes
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Three selectable accelerating voltages
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No special sample preparation, for conductive and non-conductive samples
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Sample loading to imaging in less than three minutes