Jumi A. Shin

Jumi A. Shin

Title/Position
Ph.D. | Associate Professor
Chemistry | Biological and Bioanalytical Chemistry

Research Areas:

Biological Chemistry: protein design & engineering, directed evolution, anti-cancer protein drugs, genomic mutations from nanoparticle & quantum-dot exposure.

Shin Research

 

Research Profile:

The multidisciplinary Shin lab focuses on exploiting the building blocks that nature uses, including proteins and nucleic acids, toward solving problems in human health and our environment and ecosystem. We design small proteins that bind to specific DNA targets toward regulation of gene expression, particularly those involved in cancer and disease. Protein structure and function is analyzed by various spectrosopic methods including fluorescence (FRET, anisotropy), circular dichroism and x-ray crystallography, as well as biological assays (yeast and bacterial one-hybrid) and testing in cancer cell lines and mouse models.

Nanomaterials are part of our daily lives (in our cell phones, TVs, paints and coatings, and more), so we are trying to understand how nanomaterials can exert pressure on organisms in the environment to evolve. Using a multidisciplinary approach involving quantitative genetics, nano-engineering, and molecular biology, we observed that chronic exposure to nanomaterials can cause organisms to mutate their genomes in order to survive.

Courses Taught:

CHM242 & CHM462 (undergraduate), CHM1054 & CHM1056 (graduate)

Publications

For an overview of her transdisciplinary work designing and developing peptide therapeutics https://www.biomedicine.video/medical-devices-pharmaceuticals/designing-developing-therapeutic-proteins-jumi-shin

Recent:

"Combining Rational Design and Continuous Evolution on Minimalist Proteins That Target the E-box DNA Site." I. Inamoto, I. Sheoran, S. C. Popa, M. Hussain, & J. A. ShinACS Chem. Biol., 2020, DOI: doi/10.1021/acschembio.0c00684.

"Phage Assisted Continuous Evolution (PACE): a How-to Guide for Directed Evolution."  S. C. Popa, I. Inamoto, B. W. Thuronyi, & J. A. ShinACS Omega2020, DOI: 10.1021/acsomega.0c03508. 

"The intrinsically disordered loop in the USF1 bHLHZ domain modulates its DNA-binding sequence specificity in hereditary asthma."  S. C. Popa & J. A. ShinJournal of Physical Chemistry B2019.  DOI: 10.1021/acs.jpcb.9b06719.

 "Peptide therapeutics that directly target transcription factors." Ichiro Inamoto & J. A. ShinPeptide Science2019, 111, 1-11. DOI: 10.1002/pep2.24048.  

"Inhibiting MYC binding to the E-box DNA motif by ME47 decreases tumour xenograft growth." L. C. Lustig, D. Dingar, W. Tu, C. Lourenco, M. Kalkat, I. Inamoto, R. Ponzielli, W. C. W. Chan, J. A. Shin, & L. Z. Penn. Oncogene201736, 6830-6837. DOI: 10.1038/onc.2017.275. 

"Yeast Populations Evolve to Resist CdSe Quantum Dot Toxicity." A. Strtak, S. Sathiamoorthy, P. S. Tang, K. M. Tsoi, F. Song, J. B. Anderson, W. C. W. Chan., and J. A. Shin. Bioconjugate Chemistry, 2017. DOI: 10.1021/acs.bioconjchem.7b00056.

Below article in red has been selected as HOT article! (a HOT article contains research which has been highlighted by the reviewers as being particularly interesting or particularly significant research). Download a copy from HERE

"The DNA target determines the dimerization partner selected by bHLHZ-like hybrid proteins AhrJun and ArntFos." I. Inamoto, G. Chen, & J. A. Shin.  Molecular Biosystems, 2017, DOI: 10.1039/C6MB00795C.  

"The Role of Ligand Density and Size in Mediating Quantum Dot Nuclear Transport."  P. Tang, S. Sathiamoorthy, L. Lustig, R. Ponzielli, I. Inamoto, L. Z. Penn, J. A. Shin, & W. C. W. Chan.  Small, 201410, 4182–4192. DOI: 10.1002/smll.201401056.

"Boundaries of the Origin of Replication: Creation of a pET-28a-Derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors."  S. Sathiamoorthy & J. A. Shin.  PLoS ONE20127, e47259. doi:10.1371/journal.pone.0047259.

"The bZIP Dimer Localizes at DNA Full-Sites Where Each Basic Region Can Alternately Translocate and Bind to Subsites at the Half-Site."  I. Chan, T. Al-Sarraj, H. S. Shahravan, A. V. Fedorova, & J. A. Shin.  Biochemistry201251, 6632-6643.

"FRep: a fluorescent protein-based bioprobe for in vivo detection of protein:DNA interactions."  S. H. Shahravan, I. T. Li, K. Truong, & J. A. Shin.  Analytical Chemistry201183, 9643-9650.

"Max-E47, a designed minimalist protein that targets the E-box DNA site in vivo and in vitro."  J. Xu, G. Chen, A. T. De Jong, S. H. Shahravan, & J. A. Shin. Journal of the American Chemical Society2009131, 7839-7848.

"Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactions."  G. Chen, L. M. DenBoer, & J. A. Shin.  BioTechniques200845, 295-304.