Our lab at the University of Toronto seeks to understand the role of biomechanics on genome function, most notably, how the organization, packaging and conformational dynamics of DNA affect gene expression. We develop and employ a unique complement of single-molecule instruments for force-spectroscopy and single-molecule localization microscopy (SMLM). Force-spectroscopy enables us to probe the biophysical mechanisms underlying protein-DNA interactions, while SMLM reveals information on the spatial organization, abundance and dynamics of proteins and nucleic acids, within both bacteria and eukaryotic cells, at an unprecedented level of detail.

What's Happening

(05/31/19): Daniel's paper on "Nanoscopic Stoichiometry and Single-Molecule Counting" was just published in Small Methods and is available online here.

(05/30/19): Daniel won the poster prize for single-molecule biophysics at the 2019 Biophysical Society of Canada Annual meeting.

(05/05/19): New summer ROP research students Victoria, Eric and Muhammed just started working in the lab.

(04/9/19): Jovana Miladinovic was awarded a University of Toronto Excellence Award (UTEA) to work in the lab over the summer.

(02/24/19): Nafiseh's manuscript "Growth Phase Dependent Chromosome Condensation and H-NS Protein Redistribution in E. coli Under Osmotic Stress" is now available as a preprint online.

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