Our lab at the University of Toronto seeks to understand the role of biomechanics on genome function, most notably, how the organization, packaging and conformational dynamics of DNA affect gene expression. We develop and employ a unique complement of single-molecule instruments for force-spectroscopy and single-molecule localization microscopy (SMLM). Force-spectroscopy enables us to probe the biophysical mechanisms underlying protein-DNA interactions, while SMLM reveals information on the spatial organization, abundance and dynamics of proteins and nucleic acids, within both bacteria and eukaryotic cells, at an unprecedented level of detail.
(4/1/20): We're all on hold due to COVID-19, but we'll be back in the lab as soon as we can. If you're interested in joining, just send an email to josh.milstein[at]utoronto.ca.