For Bruker 400 MHz instrument
Our Bruker Avance-III 400 instrument does not require booking.
Please inquire if you require training.
Usage requirements
- Use 7" or 8" standard 5-mm NMR tubes.
- For specialty tubes - max recommended length is 220 mm
- Use of 3-mm is possible though the use of an adaptor
- Unfortunately use of 3-mm samples with CDCL3 is not currently feasible
- Solvents like D2O, DMSO, Acetone, DCM, Benzene, Methanol are okay
- We highly recommend use of Norell SampleVault series for all your needs
- Only completely intact tubes are allowed (general facility rule)
- Solvent height is recommended to be 40 mm
- This correlates to 500 uL of solution in standard 5-mm tubes
- This correlates to 180 uL of solution in standard 3-mm tubes
- Day queue is from 10 am to 6 pm
- The queue is continuous from Friday to Monday
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Please note, we'll be switching to a new booking schedule. Here is our new booking system that is built by Book-it Labs.
As of right now, please check the calendar for our high-field spectrometer availability: 600 MHz Calendar
Please note: VnmrS and Inova correspond to the console type, please check our instrument page for list of available probes.
To book the high field spectrometers please email facility manager and CC your supervisor. Spectrometers are booked only upon explicit confirmation from the supervisor.
Only specially trained users are allowed to book time on the 500 MHz and the 600 MHz instruments.
We do offer data acquisition on these spectrometers as a service.
Here are some of the common experiments we offer:
- VT experiments between -80 deg.C and +100 deg.C (probe dependent)
- High sensitivity characterizations data (1Ds and 2Ds) for small sample quantities
- DOSY experiments
- HOESY experiments
- Ligand binding experiments
- Protein assignment data sets
- SS-NMR experiments (5-mm MAS probe up to 10kHz)
Users can be trained to perform various experiments on their own. Spectrometer availability varies, please inquire.
Processing Software
For processing you are able to use Topspin, VnmrJ, and MestReNova routinely.
Processing licenses are available only for University of Toronto affiliated users.
Licenses for MestReNova are available through facility.
License for Topspin is available through Bruker website using our spectrometer's order number
There is no license required for processing version of the VnmrJ.
To download MestReNova please go to their official site. Remeber to chose correct version and keep it up to date.
To download Topspin please go to the Bruker official site. You will have to create an account.
Additional processing software that you may find useful:
NMRFx: great for 2D, 3D, and relaxation data
CCP NMR: great for 2D, 3D, binding experiments