Updated May 2, 2017

The Imaging Facility is located in DV1074C and maintained by core facility technician, Xin Zhao, from Department of Biology. It functions as a shared research resource at UTM for optical microscopy, and is currently equipped with confocal and epi-fluorescence microscopes. In the future we hope to enhance our imaging suite through the acquisition of new microscopes based on research requirements.

Hours:

The facility can be accessed 24/7 via T-card – please contact  Xin Zhao to obtain authorization. Xin Zhao is available Monday to Friday, 8:45am to 5pm

Contact Information:

Xin Zhao
Core Facility Technician     
Department of Biology, Room DV4070
University of Toronto Mississauga
Tel:  905 569 4270
Email: xin.zhao@utoronto.ca

Facility Microscopes:

• Confocal microscope Zeiss LSM510
  • Microscope: Axioplan 2, upright
  • Laser types:
    • Diode - 405nm
    • Argon 2 - 458/477/488/514 nm
    • HeNe 1  - 543nm
    • HeNe 2 - 633nm
  • Objectives:
    • Plan-Neofluar 10x0.3 Dry
    • Plan-Apochromat 20x0.75 Dry
    • Plan-Apochromat 40x/1.0 Oil Iris
    • Plan-Apochromat 63x/1.4 Oil DIC
    • Achroplan 100x1.0 W ph3
  • Fluorescence power supply: X-Cite
  • Fluorescence filter:
    • GFP
    • TRITC
    • Dapi
    • FITC
  • Imaging software: Zen 2009
  • Camera: Non
• Epifluorescence microscope  Nikon Eclipse 50i
  • Microscope: upright, phase contrast
  • Condenser: phase contrast condenser 0.9 dry
  • Objectives:
    • Nikon Plan uw 1x/0.04
    • Nikon Plan Fluo 4x /0.13
    • Nikon Plan Fluo 10x/0.25
    • Nikon Plan  20x/0.04
    • Nikon Plan Fluo 100x1.30 Oil
  • Filters:
    • ET Dapi
    • ET GFP
    • ET RsRed
    • UV-2A
  • Fluorescence power supply: X-Cite
  • Imaging Software: NIS-Elements BR3.0
  • Camera: Nikon DS-Ri1

Facility Services:

• Free basic training on confocal microscope Zeiss LSM510 is mandatory for new users.
• Technical support and a number of customized services such as sample fixation, antibody staining, and in-situ hybridization are available from the core facility technician. Fees will be associated with some services, and the time commitment for such services will be discussed in advance with a cost of $30/hr (plus the cost of materials) for technician’s time. Please contact Xin Zhao for cost estimates and quotes.