Canadian Drosophila Microarray Centre (CDMC)
is a national microarray service for Canadian scientists working with
Drosophila. There are two main objectives of this service:
1. To produce and provide Drosophila DNA microarrays on glass slides.
2. To provide a microarray experiment service facility where the scientist
provides a sample of RNA (or DNA) to be tested and the service technician
converts it to a labelled cDNA, hybridizes it to a microarray, scans
it and performs the initial analysis.
Both the microarrays and the experiment service will be provided to
Canadian researchers for a nominal cost.
the CDMC has or intends to:
3. Provide information and protocols for the use of the Drosophila microarrays.
4. Supply individual EST clones used to make the array for a nominal
5. Support the creation and use of a Drosophila microarray experiment
is federally funded with funds coming from both National Sciences and
Engineering Research Council (Genomics grant) and the Canadian Institutes
of Health Research (Multi-User Equipment and Maintenance grant).
for Having the CDMC
With the publication of the complete, annotated Drosophila genome sequence
in March 2000, ninety years of research at the forefront of genetics
and molecular genetics has come to fruition and Drosophila research
thus moves now into the 'post-genome' era. Recent technological advances
have allowed genome-wide analysis of such phenomena as gene function,
gene expression and detection of genetic abnormalities and pathological
states. One of the most powerful and exciting of these technologies
is DNA array based parallel genome analysis, often commonly referred
to as DNA microarrays or gene chips. At present, we are aware of only
one major commercial microarray company (Affymetrix) that is selling
Drosophila microarrays (gene chips) and is currently charging approximately
$500 Cdn. per array (not including labeling reagents). To use the Affymetrix
chips one requires approximately $300,000 Cdn. of proprietary equipment.
In recent publications that have utilized microarrays, it is apparent
that most studies perform tens and in some cases hundreds of pairs of
analyses. Therefore, if Canadian scientists are to utilize microarray
technology and compete on an international level, arrays have to be
made available at a more affordable price.