Shin, Jumi A.

Ph.D. | Associate Professor | ChemistryBiological and Bioanalytical Chemistry

Contact Information

Phone: 
905 828 5355
Fax: 
905 828 5425
Room: 
DV4039
Mailing Address: 
3359 Mississauga Road
City: 
Mississauga
Province: 
Ontario
Postal Code: 
L5L 1C6
Picture of Jumi A. Shin

Research Areas:

Biological Chemistry: protein design & engineering, directed evolution, anti-cancer protein drugs, genomic mutations from nanoparticle & quantum-dot exposure.

Research Profile:

The multidisciplinary Shin lab focuses on exploiting the building blocks that nature uses, including proteins and nucleic Shin Researchacids, toward solving problems in human health and our environment and ecosystem. We design small proteins that bind to specific DNA targets toward regulation of gene expression, particularly those involved in cancer and disease. Protein structure and function is analyzed by various spectrosopic methods including fluorescence (FRET, anisotropy), circular dichroism and x-ray crystallography, as well as biological assays (yeast and bacterial one-hybrid) and testing in cancer cell lines and mouse models.

Nanomaterials are part of our daily lives (in our cell phones, TVs, paints and coatings, and more), so we are trying to understand how nanomaterials can exert pressure on organisms in the environment to evolve. Using a multidisciplinary approach involving quantitative genetics, nano-engineering, and molecular biology, we observed that chronic exposure to nanomaterials can cause organisms to mutate their genomes in order to survive.

Courses Taught:

CHM242 & CHM462 (undergraduate), CHM1054 & CHM1056 (graduate)

Publications

Recent:

"Yeast Populations Evolve to Resist CdSe Quantum Dot Toxicity." A. Strtak, S. Sathiamoorthy, P. S. Tang, K. M. Tsoi, F. Song, J. B. Anderson, W. C. W. Chan., and J. A. Shin. Bioconjugate Chemistry, 2017. DOI: 10.1021/acs.bioconjchem.7b00056.

Below article in red has been selected as HOT article! (a HOT article contains research which has been highlighted by the reviewers as being particularly interesting or particularly significant research). Download a copy from HERE

"The DNA target determines the dimerization partner selected by bHLHZ-like hybrid proteins AhrJun and ArntFos." I. Inamoto, G. Chen, & J. A. Shin.  Molecular Biosystems, 2017, DOI: 10.1039/C6MB00795C.  

"The Role of Ligand Density and Size in Mediating Quantum Dot Nuclear Transport."  P. Tang, S. Sathiamoorthy, L. Lustig, R. Ponzielli, I. Inamoto, L. Z. Penn, J. A. Shin, & W. C. W. Chan.  Small, 2014, 10, 4182–4192. DOI: 10.1002/smll.201401056.

"Boundaries of the Origin of Replication: Creation of a pET-28a-Derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors."  S. Sathiamoorthy & J. A. ShinPLoS ONE, 2012, 7, e47259. doi:10.1371/journal.pone.0047259.

"The bZIP Dimer Localizes at DNA Full-Sites Where Each Basic Region Can Alternately Translocate and Bind to Subsites at the Half-Site."  I. Chan, T. Al-Sarraj, H. S. Shahravan, A. V. Fedorova, & J. A. ShinBiochemistry, 2012, 51, 6632-6643.

"FRep: a fluorescent protein-based bioprobe for in vivo detection of protein:DNA interactions."  S. H. Shahravan, I. T. Li, K. Truong, & J. A. ShinAnalytical Chemistry, 2011, 83, 9643-9650.

"Max-E47, a designed minimalist protein that targets the E-box DNA site in vivo and in vitro."  J. Xu, G. Chen, A. T. De Jong, S. H. Shahravan, & J. A. Shin. Journal of the American Chemical Society, 2009, 131, 7839-7848.

"Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactions."  G. Chen, L. M. DenBoer, & J. A. ShinBioTechniques, 2008, 45, 295-304.