Imaging Facility

The Imaging Facility is located in DV1074C and maintained by the core facility technician, Xin Zhao, from the Department of Biology. It functions as a shared research resource at UTM for optical microscopy, and is currently equipped with confocal and epi-fluorescence microscopes. In the future we hope to enhance our imaging suite through the acquisition of new microscopes based on research requirements.

Hours:

The facility can be accessed 24/7 via T-card – please contact  Xin Zhao to obtain authorization. Xin Zhao is available Monday to Friday, 8:45am to 5pm

Contact Information:

Xin Zhao
Core Facility Technician     
Department of Biology, Room DV4005
University of Toronto Mississauga
Tel:  905 569 4270
Email: xin.zhao@utoronto.ca

Facility Microscopes:

  • Confocal microscope Zeiss LSM510
    • Microscope: Axioplan 2, upright
    • Laser types:
      • Diode - 405nm
      • Argon 2 - 458/477/488/514 nm
      • HeNe 1  - 543nm
      • HeNe 2 - 633nm
    • Objectives:
      • Plan-Neofluar 10x0.3 Dry
      • Plan-Apochromat 20x0.75 Dry
      • Plan-Apochromat 40x/1.0 Oil Iris
      • Plan-Apochromat 63x/1.4 Oil DIC
      • Achroplan 100x1.0 W ph3
    • Fluorescence power supply: X-Cite
    • Fluorescence filter:
      • GFP
      • TRITC
      • Dapi
      • FITC
    • Imaging software: Zen 2009
    • Camera: Non
  • Epifluorescence microscope  Nikon Eclipse 50i
    • Microscope: upright, phase contrast
    • Condenser: phase contrast condenser 0.9 dry
    • Objectives:
      • Nikon Plan uw 1x/0.04
      • Nikon Plan Fluo 4x /0.13
      • Nikon Plan Fluo 10x/0.25
      • Nikon Plan  20x/0.04
      • Nikon Plan Fluo 100x1.30 Oil
    • Filters:
      • ET Dapi
      • ET GFP
      • ET RsRed
      • UV-2A
    • Fluorescence power supply: X-Cite
    • Imaging Software: NIS-Elements BR3.0
    • Camera: Nikon DS-Ri1

Facility Services:

  • Free basic training on confocal microscope Zeiss LSM510 is mandatory for new users.
  • Technical support and a number of customized services such as sample fixation, antibody staining, and in-situ hybridization are available from the core facility technician. Fees will be associated with some services, and the time commitment for such services will be discussed in advance with a cost of $60/hr (plus cost of materials) for the technician’s time.  Please contact Xin Zhao for cost estimates and quotes.